6533b7d4fe1ef96bd1261d4b
RESEARCH PRODUCT
DETERMINATION OF PENTOBARBITAL IN BIOLOGICAL SAMPLES BY MICELLAR LIQUID CHROMATOGRAPHY
M. J. Medina-herníndeRosa María Villanueva-camañasYolanda Martín-bioscaSalvador Sagradosubject
Detection limitPentobarbitalChromatographyChemistryClinical BiochemistryAnalytical chemistryPharmaceutical ScienceReversed-phase chromatographyBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryMicellar liquid chromatographymedicineSample preparationSolid phase extractionQuantitative analysis (chemistry)medicine.drugdescription
A liquid chromatographic procedure for the determination of pentobarbital in urine and plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C18 analytical column, a guard column of similar characteristics, and a 0.02 M CTAB-15% 1-propanol at pH 7.5 mobile phase. The UV detector was set at 250 nm. Pentobarbital was isolated from urine and plasma samples by using a single solid phase extraction procedure with LMS cartridges. Mephobarbital was used as internal standard. Limits of detection were 0.53 μg/mL and 0.60 μg/mL in urine and plasma samples respectively. In both cases the coefficients of variation were lower than 6.5%, and the recoveries ranged between 73–105%.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-01 | Journal of Liquid Chromatography & Related Technologies |