6533b7d4fe1ef96bd1261e01
RESEARCH PRODUCT
Nucleoside phosphotransferase of chick embryo
Renza VentoGennaro TaibiGiovanni TesoriereGiuseppe Calvarusosubject
chemistry.chemical_classificationRibonucleotideClinical BiochemistrySize-exclusion chromatographyChick EmbryoCell BiologyGeneral MedicineHydrogen-Ion ConcentrationThymidine KinaseSubstrate SpecificityMolecular WeightDeoxyribonucleosidechemistry.chemical_compoundDeoxyribonucleotideEnzymeIsoelectric pointchemistryBiochemistryNucleoside phosphotransferaseChromatography GelAnimalsNucleotideMolecular Biologydescription
This paper describes a purification procedure and some properties of a nonspecific nucleoside phosphotransferase of chick embryo, an activity which catalyzes the transfer of chick embryo, an activity which catalyzes the transfer of the phosphate ester from a deoxyribonucleotide or a pyrimidine ribonucleotide to a deoxyribonucleoside acceptor. The enzyme is very unstable to heat, dilution and dialysis and it is almost entirely inactivated by DEAE-cellulose chromatography or gel filtration. A marked enhancement in its stability is caused by numerous nucleotides. In these experiments at least 920-fold purification was obtained by using dTTP (50 microM) as nucleotide protector. The enzyme, purified in presence of dTTP, has a molecular weight about 270,000, an isoelectric point of 6.27, a pH optimum of 8.8 and is stable at 37 degrees C at least for 10 min. In absence of nucleotide protector, nucleoside phosphofranserferase is connected at 37 degrees C or by gel filtration in a very small active form with a lower molecular weight (about 30,000) and a pH optimum of 7.6.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1979-06-01 | Molecular and Cellular Biochemistry |