6533b7d4fe1ef96bd1262a35
RESEARCH PRODUCT
Freezing Induces Biased Results in the Molecular Detection of Flavobacterium columnare
Marja TiirolaL.-r. SuomalainenRitva IjäsHilkka ReunanenE. Tellervo Valtonensubject
DNA BacterialApplied Microbiology and BiotechnologyFlavobacteriumPolymerase Chain ReactionBacterial geneticsMicrobiologylaw.inventionchemistry.chemical_compoundlawFreezingMethodsPathogenFalse Negative ReactionsPolymerase chain reactionEcologybiologybiology.organism_classificationFlavobacteriaceaeMicroscopy ElectronchemistryFlavobacterium columnareBacteriaDNAFlavobacteriumFood ScienceBiotechnologydescription
ABSTRACT Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-02-01 |