6533b7d4fe1ef96bd1263052
RESEARCH PRODUCT
Studies on zymogenicity and solubilization of chitin synthase from Candida albicans
Rafael SentandreuDaniel GozalboFrancisco Dubónsubject
endocrine systemmacromolecular substancesBiologydigestive systemMicrobiologychemistry.chemical_compoundZymogenGeneticsmedicineCandida albicansMolecular Biologychemistry.chemical_classificationChitin synthaseTrypsinbiology.organism_classificationMolecular biologyYeastIn vitrocarbohydrates (lipids)enzymes and coenzymes (carbohydrates)EnzymeDigitoninchemistryBiochemistrybiology.proteinmedicine.drugdescription
The zymogenic form of the chitin synthase present in mixed membrane preparations was extracted by digitonin treatment. The residual extracted membranes exclusively retained the basal activity. Trypsin activation of the zymogenic form of the enzyme did not modify the digitonin solubilization characteristics of the original zymogenic form, suggesting significant differences between ‘in vivo’ activation of chitin synthase and that carried out by trypsin ‘in vitro’.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1985-01-01 | FEMS Microbiology Letters |