6533b7d4fe1ef96bd12632af
RESEARCH PRODUCT
Transmissible gastroenteritis virus (TGEV)-based vectors with engineered murine tropism express the rotavirus VP7 protein and immunize mice against rotavirus
Rebeca MontavaJavier BuesaLuis EnjuanesJavier OrtegoJuan Manuel RibesJuan E. Cerianisubject
MaleViral vectorsRotavirusSwinevirusesRecombinant virusmedicine.disease_causeAntibodies ViralVirus ReplicationMice0302 clinical medicinefluids and secretionsRotavirusAntigens ViralCoronavirus0303 health sciencesMice Inbred BALB CProtectionvirus diseases3. Good healthAnimals SucklingSTAT1 Transcription FactorRNA ViralFemaleGenetic EngineeringGene Expression Regulation ViralDiarrheaBiologyTropismArticleRotavirus InfectionsMicrobiologyViral vectorCell Line03 medical and health sciencesMouse hepatitis virusVirologymedicineAnimalsTropism030304 developmental biologyTransmissible gastroenteritis virusRotavirus Vaccinesbiology.organism_classificationVirologyImmunizationViral replicationCapsid ProteinsImmunity Maternally-Acquired030215 immunologydescription
A coronavirus vector based on the genome of the porcine transmissible gastroenteritis virus (TGEV) expressing the rotavirus VP7 protein was constructed to immunize and protect against rotavirus infections in a murine model. The tropism of this TGEV-derived vector was modified by replacing the spike S protein with the homologous protein from mouse hepatitis virus (MHV). The rotavirus gene encoding the VP7 protein was cloned into the coronavirus cDNA. BALB/c and STAT1-deficient mice were inoculated with the recombinant viral vector rTGEVS-MHV-VP7, which replicates in the intestine and spreads to other organs such as liver, spleen and lungs. TGEV-specific antibodies were detected in all the inoculated BALB/c mice, while rotavirus-specific antibodies were found only after immunization by the intraperitoneal route. Partial protection against rotavirus-induced diarrhea was achieved in suckling BALB/c mice born to dams immunized with the recombinant virus expressing VP7 when they were orally challenged with the homotypic rotavirus strain.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-02-01 |