6533b7d4fe1ef96bd12632ce

RESEARCH PRODUCT

Enhancement of immunohistochemical staining

P J McmillanP J Mcmillan

subject

Blood Specimen CollectionChromatographyStaining and LabelingHistocytochemistryChemistryGoatsImmunochemistryThyroid GlandMixing (process engineering)Plastic filmGeneral MedicineEvaporation (deposition)RatsVolume (thermodynamics)CalcitoninAnimalsImmunohistochemistryRabbitsAnatomyGeneral Agricultural and Biological Sciences

description

The present paper describes two simple procedures which enhance immunohistochemical staining. One is to cover the sections with a plastic film to keep the serum uniformly distributed and minimize its evaporation. Secondly, rocking of the slides has been introduced, causing the serum to flow back and forth under the plastic film. Using this system, it has been possible to test for the effect of mixing on an immunohistochemical reaction (the demonstration of calcitonin in thyroid C cells). It has been found that mixing definitely enhances the reaction during the first 8 h. No effect of serum volume was observed.

yearjournalcountryeditionlanguage
1982-06-01Histochemistry
https://doi.org/10.1007/bf00496011