6533b7d5fe1ef96bd1263d53
RESEARCH PRODUCT
Hemoglobin loaded polymeric nanoparticles: preparation and characterizations.
Federica ChielliniAntonio CupaneAnna Maria PirasAlberto DessyGiorgio SchiròMatteo Levantinosubject
Biocompatible polymerVinyl CompoundsInjectable systemsBiocompatibilityReducing agentPharmaceutical ScienceNanoparticleBiocompatible MaterialsPolymeric nanoparticlePolyethylene Glycolschemistry.chemical_compoundHemoglobinsBlood SubstitutesPolymer chemistrymedicineHumansMicroparticleParticle SizeMaleic AnhydridesDrug CarriersBlood substituteMaleic anhydrideVinyl etherSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)chemistryChemical engineeringNanoparticlesEthylene GlycolsDrug carrierEthylene glycolmedicine.drugEthersdescription
In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138 ± 10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences |