6533b7d5fe1ef96bd1263dad

RESEARCH PRODUCT

The biological activity of bacteriophage DNA, prepared by the cationic detergent dilution technique

Rudolf K. ZahnGerhard Seibert

subject

DetergentsViral Plaque AssayBiologyVirus Replicationmedicine.disease_causeColiphagesBacteriophagechemistry.chemical_compoundEscherichia coliGeneticsmedicinePhenolEscherichia coliVirus quantificationDNA VirusesBiological activityLambda phageChromatography Ion Exchangebiology.organism_classificationMolecular biologyQuaternary Ammonium CompoundschemistryBiochemistryViral replicationDNA ViralDNA

description

Abstract The preparation of phage lambda DNA infecting E. coli K 12 with cationic detergent is described. This DNA infects E. coli spheroblasts with the same efficiency as DNA prepared by phenol methods.

https://doi.org/10.1093/nar/2.3.347