6533b7d5fe1ef96bd1263f08
RESEARCH PRODUCT
Glycoprotein and ganglioside changes in human trophoblasts after exposure to pulsed doppler ultrasound
Mirna FlögelBranko BreyerMarija Heffer-laucGordan LaucGordan LaucVišnja LatinWerner E.g. Müllersubject
Acoustics and UltrasonicsImmunoblottingBiophysicsMannoseG(M2) Gangliosidechemistry.chemical_compoundGangliosidesLectinsmedicineG(M3) GangliosideHumansRadiology Nuclear Medicine and imagingGlycoproteinschemistry.chemical_classificationMembrane GlycoproteinsGangliosideGalanthusRadiological and Ultrasound TechnologybiologyChemistryTrophoblastLectinMolecular biologyN-Acetylneuraminic AcidTrophoblastsMolecular WeightMembrane glycoproteinsglycoproteins; gangliosides; lectins; pulsed doppler ultrasoundmedicine.anatomical_structureUltrasonography Doppler PulsedG(M2) GangliosideImmunologySialic Acidsbiology.proteinElectrophoresis Polyacrylamide GelChromatography Thin LayerPlant LectinsGlycoproteinMannoseN-Acetylneuraminic aciddescription
Changes in glycoprotein and ganglioside composition in human trophoblasts (eighth week of gestation) after in vitro exposure to pulsed Doppler ultrasound (pulse duration 1.22 microseconds; repetition frequency 11.1 kHz; center frequency 4 MHz; ISPPA = 175.5 W/cm2; ISPTA = 0.59 W/cm2) were investigated. Evacuated trophoblasts were divided in two halves and insonated for 10 min on top of a 6-cm layer of 5% gelatin in 50-mL tubes (Falcon) at 37 degrees C. One half of each trophoblast was sham insonated and served as an internal control. After insonation trophoblasts were maintained at 37 degrees C for 24 h. Glycoproteins were detected using alpha-D-mannose specific lectins from Galanthus nivalis and Narcissus pseudonarcissus. A decrease in the expression of mannose containing glycoprotein mgp47 and an increase in expression of mgp54 were observed. Ganglioside composition was also significantly altered. Concentrations of two gangliosides migrating similarly to GM2, and one similarly to GQ1, decreased by more than 75%. At the same time, concentrations of one ganglioside migrating similarly to GM3, and two other unidentified gangliosides increased two- to fourfold.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-01-01 |