6533b7d5fe1ef96bd12645aa
RESEARCH PRODUCT
Determination of theophylline and paraxanthine in urine samples by liquid chromatography using the H-point standard additions method
Adela Sevillano-cabezaFrancisco Bosch-reigRosa Herráez-hernándezPilar Campíns-falcósubject
ChromatographyElutionExtraction (chemistry)UrineBiochemistryAnalytical Chemistrychemistry.chemical_compoundchemistryStandard additionmedicineEnvironmental ChemistryGradient elutionTheophyllineSolvent extractionSpectroscopyParaxanthinemedicine.drugdescription
Abstract The simultaneous determination of theophylline and paraxanthine in urine samples by the H-point standard additions method (HPSAM) is described. Samples are extracted with C 18 solid-phase extraction cartridges and chromatographed using a Hypersil C 18 -ODS column and a mobile phase consisting of acetonirile-phosphate buffer in the gradient elution mode. Under these conditions theophylline and paraxanthine are eluted with short retention times. Although their chromatographic peaks are overlapped and their spectra are very similar, the H-point standard additions method provides excellent results in the determination of both xanthines at therapeutic levels.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-10-01 | Analytica Chimica Acta |