6533b7d5fe1ef96bd126485a
RESEARCH PRODUCT
An open-source computational and data resource to analyze digital maps of immunopeptidomes
David S. CampbellRobert L. MoritzEtienne CaronSri H. RamarathinamHeiko SchusterRuedi AebersoldRalf B. SchittenhelmMiguel MarcillaPedro NavarroNicola TernetteTheo SturmEric W. DeutschSangtae KimCecilia S. Lindestam ArlehamnDaniel J. KowalewskiAnthony W. PurcellHans-georg RammenseeStefan StevanovicLudovic C GilletHenry H N LamLucia EsponaAdán AlpízarArmin RabsteynChing C G KohAlessandro Settesubject
Databases FactualimmunopeptidomeQH301-705.5Systems biologyScienceImmunologyComputational biologyBiologyBioinformaticsMass spectrometryGeneral Biochemistry Genetics and Molecular BiologyMass Spectrometry03 medical and health sciencesResource (project management)Fragment (logic)HLA Antigenstargeted mass spectrometryHigh-Throughput Screening AssaysDIAhumanAntigenshuman leukocytes antigenBiology (General)030304 developmental biology0303 health sciencesAntigen PresentationGeneral Immunology and MicrobiologyDigital mappingGeneral Neuroscience030302 biochemistry & molecular biologyQRComputational BiologyGeneral Medicine3. Good healthTools and ResourcesHigh-Throughput Screening AssaysWorkflowTargeted mass spectrometrySWATH-MSMedicinePeptidesComputational and Systems Biologydescription
We present a novel mass spectrometry-based high-throughput workflow and an open-source computational and data resource to reproducibly identify and quantify HLA-associated peptides. Collectively, the resources support the generation of HLA allele-specific peptide assay libraries consisting of consensus fragment ion spectra, and the analysis of quantitative digital maps of HLA peptidomes generated from a range of biological sources by SWATH mass spectrometry (MS). This study represents the first community-based effort to develop a robust platform for the reproducible and quantitative measurement of the entire repertoire of peptides presented by HLA molecules, an essential step towards the design of efficient immunotherapies. DOI: http://dx.doi.org/10.7554/eLife.07661.001
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-07-01 | eLife |