6533b7d5fe1ef96bd12649af

RESEARCH PRODUCT

Identification of DNA sequences specific for Vibrio vulnificus biotype 2 strains by suppression subtractive hybridization.

Carmen AmaroLien-i HorEva SanjuánChung-te Lee

subject

Molecular Sequence DataVibrio vulnificusApplied Microbiology and BiotechnologyPolymerase Chain ReactionDNA sequencinglaw.inventionMicrobiologyNucleic acid thermodynamicsFish DiseasesPlasmidSpecies SpecificitylawMethodsAnimalsHumansSerotypingVibrio vulnificusPolymerase chain reactionGeneticsEelsEcologybiologyBase SequenceVirulenceNucleic acid sequenceNucleic Acid Hybridizationbiology.organism_classificationgenomic DNASuppression subtractive hybridizationVibrio InfectionsFood ScienceBiotechnologyPlasmids

description

ABSTRACT Vibrio vulnificus can be divided into three biotypes, and only biotype 2, which is further divided into serovars, contains eel-virulent strains. We compared the genomic DNA of a biotype 2 serovar E isolate (tester) with the genomic DNAs of three biotype 1 strains by suppression subtractive hybridization and then tested the distribution of the tester-specific DNA sequences in a wide collection of bacterial strains. In this way we identified three plasmid-borne DNA sequences that were specific for biotype 2 strains irrespective of the serovar and three chromosomal DNA sequences that were specific for serovar E biotype 2 strains. These sequences have potential for use in the diagnosis of eel vibriosis caused by V. vulnificus and in the detection of biotype 2 serovar E strains.

10.1128/aem.71.9.5593-5597.2005https://pubmed.ncbi.nlm.nih.gov/16151155