6533b7d5fe1ef96bd12651e8

RESEARCH PRODUCT

Capillary electrophoresis–inductively coupled plasma-mass spectrometry hyphenation for the determination at the nanogram scale of metal affinities and binding constants of phosphorylated ligands

Mélanie BourdillonMichel MeyerYi LinAlain WagnerFrédéric TaranRachid BaatiMarie BrellierAgnès HagègeEric DorisFanny VarenneLoïc J. Charbonnière

subject

Formateschemistry.chemical_elementLigandsLanthanoid Series ElementsBiochemistryMass SpectrometryAnalytical ChemistryMetalchemistry.chemical_compoundCapillary electrophoresisEuropiumLimit of DetectionInductively coupled plasma mass spectrometryPrincipal Component AnalysisChromatographySodium formateMethanolOrganic ChemistryElectrophoresis CapillaryReproducibility of ResultsGeneral MedicinePhosphorus Compoundschemistryvisual_artLinear Modelsvisual_art.visual_art_mediumThermodynamicsSpectrophotometry UltravioletTitrationAbsorption (chemistry)EuropiumPhosphine

description

Abstract A screening strategy based on hyphenated capillary electrophoresis and inductively coupled plasma mass spectrometry (CE–ICP-MS) was developed to classify phosphorylated ligands according to their europium(III) binding affinity in a hydro-organic medium (sodium formate, pH 3.7, H2O/MeOH 90:10, v/v). Taking advantage of the high sensibility of ICP-MS for detecting phosphorus, this method enabled to assess the affinity of a variety of phosphorylated compounds, including phosphine oxides, thiophosphines, phosphonates, and phosphinates, in less than 1 h and using less than 5 ng of substance. By varying the total europium concentration, complexation constants could be determined according to a sequential multiple run strategy, which proved to be in excellent agreement with the values obtained by UV–Vis absorption spectrophotometric titrations.

yearjournalcountryeditionlanguage
2012-03-01Journal of Chromatography A
https://doi.org/10.1016/j.chroma.2012.01.066