6533b7d6fe1ef96bd126572d
RESEARCH PRODUCT
Characterization of transfected HT-29 cells expressing the oncogenic Ras isoform KrasG13D.
Maurizio BellaviaMario La FarinaPaolo ColombaVincenzo EternoFabrizio MirandaIda Albanesesubject
HT-29proliferationSettore BIO/06 - Anatomia Comparata E CitologiaK-radescription
Point mutations in codon 12 and 13 of K-ras are frequently found in DNA of colorectal cancer. It has been suggested that particular mutations at these sites may be associated with specific tumour phenotypes. To shed light on the molecular mechanisms on which depends this specificity we set up a system of HT-29 cells stably transfected with a cDNA coding for K-rasG13D under the control of an inducible promoter. Proliferation assay performed on one of the positives clones, showed a decreased growth rate in response to K-rasG13D expression and preliminary gene expression analysis showed an up-regulation of the cell-cycle inhibitor p21 WAF1.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 |