6533b7d6fe1ef96bd1265b41
RESEARCH PRODUCT
PARTIAL SEQUENCING OF THE BETA-GLUCOSIDASE-ENCODING GENE FROM YEAST STRAINS ISOLATED FROM MUSTS AND WINES
Rosa PalmeriCinzia Lucia RandazzoRiccardo N. BarbagalloPaolo GiudiciGiovanni SpagnaAnna Maria PugliaCristina RestucciaCinzia CaggiaPaola QuatriniSandra Marineosubject
GeneticsBETA-GLUCOSIDASEbiologyPichia anomalaAscomycotaGENE PARTIAL SEQUENCINGSaccharomyces cerevisiaeSICILIAN MUSTSbiology.organism_classificationHanseniasporaSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and BiotechnologyYeastGENE PARTIAL SEQUENCING; BETA-GLUCOSIDASE; SICILIAN MUSTSDNA glycosylaseβ-glucosidase gene partial sequencing Hanseniaspora uvarum Pichia anomala Saccharomyces cerevisiaeGenePichiadescription
The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the first time the presence of a β-glucosidase encoding gene inHanseniaspora uvarum andSaccharomyces cerevisiae.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-09-01 |