6533b7d6fe1ef96bd1266526

RESEARCH PRODUCT

Description of the retention behaviour in micellar liquid chromatography as a function of pH, surfactant and modifier concentration

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Abstract Micellar liquid chromatography permits the elution of solutes of diverse polarity. One of the most outstanding advantages of the technique is its capability of predicting the retention with high accuracy, as a function of different experimental variables. The separation of a group of compounds is usually optimized by varying the concentrations of surfactant and modifier in the mobile phase. The pH is, however, for many solutes, a variable that should be considered in the description of their elution behaviour. A global model that takes into account, simultaneously, the concentrations of surfactant and modifier, and the pH as chromatographic variables, is proposed for ionizable solutes. The mean relative errors obtained in the prediction of the retention of seven compounds eluted with 81 mobile phases of sodium dodecyl sulphate (SDS) and 1-propanol, using equations fitted with the experimental data measured in 9 to 12 mobile phases was, usually, lower than 6%. The ranges of the variables considered were 0.05–0.15 M SDS, 0–0.08 (v/v) 1-propanol and pH 3–7.