Free Chlorine and Peroxynitrite Alter the Capsid Structure of Human Norovirus GII.4 and Its Capacity to Bind Histo-Blood Group Antigens

6533b7d6fe1ef96bd126679a

RESEARCH PRODUCT

Free Chlorine and Peroxynitrite Alter the Capsid Structure of Human Norovirus GII.4 and Its Capacity to Bind Histo-Blood Group Antigens

Manon Chassaing Manon Chassaing Guillaume Bastin Maëlle Robin Didier Majou Gaël Belliot Gaël Belliot Alexis De Rougemont Alexis De Rougemont Nicolas Boudaud Christophe Gantzer

subject

Microbiology (medical)viral protein Viral protein [SDV]Life Sciences [q-bio]viruses lcsh:QR1-502 norovirus chemistry.chemical_element virus-like particles medicine.disease_cause Microbiology lcsh:Microbiology peroxynitrite Microbiology Blood group antigens 03 medical and health sciences chemistry.chemical_compound Antigen medicine Chlorine Original Research 030304 developmental biology Norovirus GII 0303 health sciences 030306 microbiology Chemistry virus diseases free chlorine biochemical phenomena metabolism and nutrition 3. Good health Capsid [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Norovirus histo-blood group antigens Peroxynitrite

description

Human noroviruses (HuNoVs) are one of the leading causes of acute gastroenteritis worldwide. HuNoVs are frequently detected in water and foodstuffs. Free chlorine and peroxynitrite (ONOO−) are two oxidants commonly encountered by HuNoVs in humans or in the environment during their natural life cycle. In this study, we defined the effects of these two oxidants on GII.4 HuNoVs and GII.4 virus-like particles (VLPs). The impact on the capsid structure, the major capsid protein VP1 and the ability of the viral capsid to bind to histo-blood group antigens (HBGAs) following oxidative treatments were analyzed. HBGAs are attachment factors that promote HuNoV infection in human hosts. Overall, our results indicate that free chlorine acts on regions involved in the stabilization of VP1 dimers in VLPs and affects their ability to bind to HBGAs. These effects were confirmed in purified HuNoVs. Some VP1 cross-links also take place after free chlorine treatment, albeit to a lesser extent. Not only ONOO− mainly produced VP1 cross-links but can also dissociate VLPs depending on the concentration applied. Nevertheless, ONOO− has less effect on HuNoV particles.

year	journal	country	edition	language
2021-04-13	Frontiers in Microbiology

https://doi.org/10.3389/fmicb.2021.662764