6533b7d6fe1ef96bd126686b

RESEARCH PRODUCT

Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco

Hélène Bracq-dieyeMarc LecuitMarc LecuitJosé Miguel SorianoMohammed El MaadoudiJamal AbriniNadia Skalli SenhajiAlexandre LeclercqAyoub KounnounAlexandra MouraNadia Amajoud

subject

0301 basic medicinemedicine.drug_classListeriaPCR-Serogrouping030106 microbiologyAntibioticsPrevalenceBiologymedicine.disease_causeMicrobiology03 medical and health sciencesListeria monocytogenes[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseasesmedicineComputingMilieux_MISCELLANEOUS2. Zero hungerGel electrophoresisbusiness.industryPFGEbiology.organism_classificationFood safety[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyMorocco030104 developmental biology[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyFood productsListeriaMultilocus sequence typingcgMLSTbusiness[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyFood ScienceBiotechnology

description

A total of 1096 food samples were purchased in Tetouan, North-Western of Morocco, to examine the presence of Listeria spp. Eighty (7.3%) of the tested samples were found positive for the presence of Listeria spp., while L. monocytogenes was detected in 16 (1.5%) samples. L. monocytogenes isolates belonged to clonal complexes CC2 (PCR serogroup IVb) and CC199 (PCR serogroup Ila) and to 8 different combined AscI/Apal pulsed-field gel electrophoresis profiles. Core genome multilocus sequence typing (cgMLST) allowed to distinguish 12 different cgMLST types and revealed the presence of 3 clusters of closely-related isolates from different samples, suggesting the existence of common sources of contamination. Isolates showed no resistance to the reference antibiotics used for the treatment of listeriosis. This study underlines the circulation of L. monocytogenes strains of clonal complex CC2 in Morocco and highlights the importance of microbiological surveillance in order to minimize consumers' exposure to this foodborne pathogen. (C) 2017 Elsevier Ltd. All rights reserved.

yearjournalcountryeditionlanguage
2018-02-01
https://fundanet.iislafe.san.gva.es/publicaciones/ProdCientif/PublicacionFrw.aspx?id=12188