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RESEARCH PRODUCT

Maritime Pine Pinus Pinaster Aiton

Isabel Mendoza-poudereuxMaría Julia Machado CanoAngeles MorcilloAlex AlborchAlicia HumánezIsabel ArrillagaJuan Segura

subject

0106 biological sciences0301 basic medicineSomatic embryogenesisVegetative reproductionfungifood and beveragesContext (language use)Biologybiology.organism_classification01 natural sciencesAcclimatizationMediterranean Basin03 medical and health sciences030104 developmental biologyGerminationBotanyPinus pinaster010606 plant biology & botanyExplant culture

description

Maritime pine (Pinus pinaster Aiton) is the most abundant conifer in the Mediterranean basin. Currently, maritime pine is considered to be a model conifer species for study of the adaption responses to drought stress from a genomics approach. In this context, the availability of protocols that allow not only mass vegetative propagation of selected families or genotypes, but also facilitate the functional analyses needed to verify and further to study the effects of candidate genes are necessary. Here we describe an improved protocol to generate maritime pine plants through somatic embryogenesis from immature megagametophytes. Accurate procedures for explant preparation, somatic embryo induction, proliferation, cryopreservation, maturation, germination, plant formation, and acclimatization are described.

yearjournalcountryeditionlanguage
2018-01-01
https://doi.org/10.1007/978-3-319-89483-6_13