6533b7d6fe1ef96bd1266f98

RESEARCH PRODUCT

Induction of CD4+/CD25+ regulatory T cells by targeting of antigens to immature dendritic cells

Alexander EnkJuergen KnopYingjie QianKarsten Mahnke

subject

CD4-Positive T-LymphocytesOvalbuminT-LymphocytesImmunologyCD8-Positive T-LymphocytesDermatitis ContactLymphocyte ActivationBiochemistryMiceInterleukin 21Antigens CDHypersensitivityAnimalsCytotoxic T cellCTLA-4 AntigenHypersensitivity DelayedLymphocyte CountIL-2 receptorAntigensAntigen-presenting cellAntigen PresentationMice Inbred BALB CCD40biologyAntibodies MonoclonalReceptors Interleukin-2Dendritic CellsCell BiologyHematologyDendritic cellNatural killer T cellAntigens DifferentiationCell biologyImmunologyInterleukin 12biology.proteinInterleukin-2Haptens

description

AbstractCoupling of ovalbumin (OVA) to anti–DEC-205 monoclonal antibody (mAb) (αDEC) induced the proliferation of OVA-specific T cells in vivo. Expansion was short-lived, caused by dendritic cells (DCs), and rendered T cells anergic thereafter. Phenotypic analysis revealed the induction of CD25+/CTLA-4+ T cells suppressing proliferation and interleukin-2 (IL-2) production of effector CD4+ T cells. The findings were supported by 2 disease models: (1) CD4+ T-cell–mediated hypersensitivity reactions were suppressed by the injection of αDEC-OVA and (2) the application of hapten-coupled αDEC-205 reduced CD8+ T-cell–mediated allergic reactions. Thus, targeting of antigens to immature DCs through αDEC antibodies led to the induction of regulatory T cells, providing the basis for novel strategies to induce regulatory T cells in vivo.

yearjournalcountryeditionlanguage
2003-06-15Blood
https://doi.org/10.1182/blood-2002-10-3229