6533b7d6fe1ef96bd12670e8

RESEARCH PRODUCT

The Candida albicans UBI3 gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth.

Daniel GozalboPatricia Roig

subject

Ribosomal ProteinsbiologyBase SequenceRecombinant Fusion ProteinsMolecular Sequence DataRibosome biogenesisGeneral Medicinebiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyFusion proteinMolecular biologyCorpus albicansPlasmidUbiquitinEssential geneCandida albicansbiology.proteinCloning MolecularCandida albicansPromoter Regions GeneticGeneRibosomesUbiquitinsPlant Proteins

description

We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes a ubiquitin fusion protein involved in ribosome biogenesis. A one-step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain (UBI3/Deltaubi3::hisG), previously isolated in the first step of the ura-blaster protocol. Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans.

yearjournalcountryeditionlanguage
2003-04-19FEMS yeast research
10.1111/j.1567-1364.2002.tb00065.xhttps://pubmed.ncbi.nlm.nih.gov/12702318