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RESEARCH PRODUCT
Synaptic release of zinc from brain slices: factors governing release, imaging, and accurate calculation of concentration.
Frederickson Christopher JGiblin Leonard JBalaji Rengarajan VRengarajan BalajiMasalha RafikFrederickson Cathleen JZeng YapingLopez Emilio VareaKoh Jae-youngChorin UdiBesser LimorHershfinkel MichalLi YangThompson Richard BKrezel Artursubject
Diagnostic ImagingPyridinesColoring agentschemistry.chemical_elementZincIn Vitro TechniquesRats Sprague-DawleyPregnancyAnimalsAcido edeticoPolycyclic CompoundsRats WistarColoring AgentsEdetic AcidFluorescent DyesNeuronsExtramuralChemistryGeneral NeuroscienceTemperatureBrainOriginal dataRatsSprague dawleyZincDentate GyrusMossy Fibers HippocampalSynapsesFemaleSynaptic VesiclesNeurosciencedescription
Cerebrocortical neurons that store and release zinc synaptically are widely recognized as critical in maintenance of cortical excitability and in certain forms of brain injury and disease. Through the last 20 years, this synaptic release has been observed directly or indirectly and reported in more than a score of publications from over a dozen laboratories in eight countries. However, the concentration of zinc released synaptically has not been established with final certainty. In the present work we have considered six aspects of the methods for studying release that can affect the magnitude of zinc release, the imaging of the release, and the calculated concentration of released zinc. We present original data on four of the issues and review published data on two others. We show that common errors can cause up to a 3000-fold underestimation of the concentration of released zinc. The results should help bring consistency to the study of synaptic release of zinc.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-06-01 | Journal of neuroscience methods |