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RESEARCH PRODUCT

Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos

Elke F RooversDavid RosenkranzMahdi MahdipourChung-ting HanNannan HeSusana M Chuva De Sousa LopesLucette A J Van Der WesterlakenHans ZischlerFalk ButterBernard A J RoelenRené F KettingLs Voortplanting Inwendige ZiektenSub Celbiologisch Lab.Es/fah BrcFertility & Reproduction

subject

MaleTransposable elementendocrine systemEmbryonic DevelopmentPiwi-interacting RNAOvaryMacaqueGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciences0302 clinical medicinebiology.animalTestismedicineAnimalsHumansRNA MessengerRNA Small Interferinglcsh:QH301-705.5030304 developmental biologyGenetics0303 health sciencesbiologyurogenital systemOvaryEmbryogenesisRNAEmbryoGerm Cellsmedicine.anatomical_structurelcsh:Biology (General)Argonaute ProteinsProteomeOocytesCattleFemale030217 neurology & neurosurgery

description

SummaryGerm cells of most animals critically depend on piRNAs and Piwi proteins. Surprisingly, piRNAs in mouse oocytes are relatively rare and dispensable. We present compelling evidence for strong Piwi and piRNA expression in oocytes of other mammals. Human fetal oocytes express PIWIL2 and transposon-enriched piRNAs. Oocytes in adult human ovary express PIWIL1 and PIWIL2, whereas those in bovine ovary only express PIWIL1. In human, macaque, and bovine ovaries, we find piRNAs that resemble testis-borne pachytene piRNAs. Isolated bovine follicular oocytes were shown to contain abundant, relatively short piRNAs that preferentially target transposable elements. Using label-free quantitative proteome analysis, we show that these maturing oocytes strongly and specifically express the PIWIL3 protein, alongside other, known piRNA-pathway components. A piRNA pool is still present in early bovine embryos, revealing a potential impact of piRNAs on mammalian embryogenesis. Our results reveal that there are highly dynamic piRNA pathways in mammalian oocytes and early embryos.

yearjournalcountryeditionlanguage
2015-03-01Cell Reports
https://doi.org/10.1016/j.celrep.2015.02.062