6533b7d6fe1ef96bd126723d

RESEARCH PRODUCT

Comparison of UV and charged aerosol detection approach in pharmaceutical analysis of statins

Dagmar SolichováDalibor ŠAtínskýLucie NovákováPetr SolichSofía Arnal LopézBohumila KulichovaAleš Horna

subject

AerosolsSimvastatinAccuracy and precisionChromatographyChemistryFormic acidDetectorAnalytical chemistryLinearityCADHigh-performance liquid chromatographyAnalytical Chemistrychemistry.chemical_compoundEquipment and SuppliesHeptanoic AcidsWide dynamic rangeAtorvastatinPyrrolesLovastatinHydroxymethylglutaryl-CoA Reductase InhibitorsQuantitative analysis (chemistry)Chromatography High Pressure LiquidTablets

description

Abstract CAD (charged aerosol detector) has recently become a new alternative detection system in HPLC. This detection approach was applied in a new HPLC method for the determination of three of the major statins used in clinical treatment—simvastatin, lovastatin and atorvastatin. The method was optimized and the influence of individual parameters on CAD response and sensitivity was carefully studied. Chromatography was performed on a Zorbax Eclipse XDB C18 (4.6 mm × 75 mm, 3.5 μm), using acetonitrile and formic acid 0.1% as mobile phase. The detection was performed using both CAD (20 pA range) and DAD (diode array detector—238 nm) simultaneously connected in series. In terms of linearity, precision and accuracy, the method was validated using tablets containing atorvastatin and simvastatin. The CAD is designated to be a non-linear detector in a wide dynamic range, however, in this application and in the tested concentration range its response was found to be perfectly linear. The limits of quantitation (0.1 μg/ml) were found to be two times lower than those of UV detection.

yearjournalcountryeditionlanguage
2009-05-15Talanta
https://doi.org/10.1016/j.talanta.2008.12.057