6533b7d7fe1ef96bd1267931
RESEARCH PRODUCT
Influence of Microspore Development on Pollen Protoplast Isolation in Digitalis obscura
Estrella ArnaltePedro Pérez-bermúdezJuan SeguraM. J. Cornejosubject
SucrosebiologyPhysiologyfungiDigitalis obscurafood and beveragesPlant ScienceCellulasebiochemical phenomena metabolism and nutritionProtoplastequipment and suppliesbiology.organism_classificationmedicine.disease_causeCell wallchemistry.chemical_compoundchemistryMicrosporePollenBotanybiology.proteinmedicinebacteriaAgaroseAgronomy and Crop Sciencedescription
Summary Protocols for the enzymatic isolation of Digitalis obscura pollen protoplasts are described. The developmental stage of the microspores was a critical factor for successful isolation since protoplasts were obtained only from tetrads. The highest yield of viable protoplasts was achieved with an enzymatic mixture containing helicase, cellulase and pectolyase in 0.5 M sucrose. In different culture techniques employed, cell wall regeneration took place only when protoplasts were plated in agarose drops.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-09-01 | Journal of Plant Physiology |