6533b7d7fe1ef96bd1267bea
RESEARCH PRODUCT
Co-expression of heat sensitive vanilloid receptor subtypes in rat dorsal root ganglion neurons
Stefan SchwarzSigrid Saaler-reinhardtWolfgang GreffrathRolf-detlef TreedeUta Binzensubject
NeuronsHot TemperatureReceptors DrugGeneral NeuroscienceTRPV2ImmunocytochemistryCentral nervous systemTRPV1TRPV Cation ChannelsBiologySpinal cordRatsRats Sprague-DawleyElectrophysiologymedicine.anatomical_structureGene Expression Regulationnervous systemDorsal root ganglionGanglia SpinalmedicineBiophysicsAnimalsNeuronNeurosciencedescription
Expression of the heat sensitive cation channels TRPV1 and TRPV2 was investigated by immunofluorescence in rat dorsal root ganglion (DRG) neurons. TRPV1-positive neurons were more frequent and had smaller diameters than TRPV2-positive neurons (35.7% vs 7.3%; 22.3 microm vs 27.6 microm), but size distributions overlapped and significant co-expression was seen in 20.7% of TRPV2-positive neurons (1.7% of all). Expression patterns did not differ between tissue sections typically used in immunocytochemistry and dissociated DRG neurons typically used in electrophysiology. Rectangular temperature pulses revealed two patterns of heat-evoked inward currents in small DRG neurons: low-threshold rapidly activating and high-threshold slowly activating. Slowly activating heat-evoked currents have not been described previously, but correspond to the type I heat responses of primary nociceptive afferents, which have been suggested to be mediated by TRPV2.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-12-01 | NeuroReport |