6533b7d7fe1ef96bd12685aa
RESEARCH PRODUCT
Real-time PCR detection of Ochroconis lascauxensis involved in the formation of black stains in the Lascaux Cave, France
Cesáreo Sáiz-jiménezFabiola BastianClaude AlabouvettePedro M. Martin-sanchezsubject
Environmental Engineering[SDV]Life Sciences [q-bio]Pcr assayFungal outbreaksFungusUnesco world heritageReal-Time Polymerase Chain Reactionlaw.inventionMicrobiology03 medical and health sciencesAscomycotaCavelaw[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyEnvironmental Chemistry[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyColoring AgentsDNA FungalWaste Management and Disposal[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/MycologyPolymerase chain reactionDNA Primers030304 developmental biology0303 health sciencesgeographygeography.geographical_feature_categoryBase Sequencebiology030306 microbiologyEcologyLascaux CaveOchroconis lascauxensisbiology.organism_classification[SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/MycologyPollution3. Good healthgenomic DNAReal-time polymerase chain reactionOchroconis lascauxensis[SDE]Environmental SciencesFranceReal-time PCRdescription
A real-time Polymerase Chain Reaction (PCR) assay was developed to detect and quantify Ochroconis lascauxensis in the Lascaux Cave in France. This fungus is the principal causal agent of the black stains threatening the Paleolithic paintings of this UNESCO World Heritage Site. The black stains outbreak could not be stopped in spite of using intensive biocide treatments. A sensitive and time-saving protocol is needed for determining the extent of the colonization. Sets of primers that target the ITS and RPB2 regions were designed and evaluated for specificity against O. lascauxensis. Genomic DNA extracted from five species of Ochroconis and 13 other fungal species frequently isolated from caves were used to test the specificity of each primer set. The specific and sensitive real-time PCR assay using the primers 347F/493R targeting a 147-bp fragment from the RPB2 gene was useful for quantifying the presence of O. lascauxensis in the stains on the walls, sediments and air of the cavity. The results confirmed the association of this fungus with the black stains and its wide dissemination in all cave compartments. The suitability of this method for monitoring fungal outbreaks in cave environments is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-08-24 | Science of The Total Environment |