6533b7d7fe1ef96bd126869d

RESEARCH PRODUCT

Identification of Patulin from Penicillium coprobium as a Toxin for Enteric Neurons

Malena Dos Santos GuilhermeNicolai M StoyeEckhard ThinesKristina EndresJulia C BaumgaertnerBenjamin BrandVu Thu Thuy NguyenAnja Schüffler

subject

Central nervous systemPharmaceutical SciencemicrobiomeBiologymedicine.disease_causeAnalytical ChemistryMicrobiologyPatulinlcsh:QD241-44103 medical and health sciencesPolyketidechemistry.chemical_compound0404 agricultural biotechnologyenteric nervous systemlcsh:Organic chemistrymycotoxinsDrug DiscoverymedicineMicrobiomePhysical and Theoretical Chemistryfusarium030304 developmental biologyCalcium signaling0303 health sciencesToxinOrganic Chemistry04 agricultural and veterinary sciences040401 food science<i>Penicillium</i>medicine.anatomical_structurechemistryChemistry (miscellaneous)Cell cultureMolecular Medicinegastrointestinal systemEnteric nervous systemfungi

description

The identification and characterization of fungal commensals of the human gut (the mycobiota) is ongoing, and the effects of their various secondary metabolites on the health and disease of the host is a matter of current research. While the neurons of the central nervous system might be affected indirectly by compounds from gut microorganisms, the largest peripheral neuronal network (the enteric nervous system) is located within the gut and is exposed directly to such metabolites. We analyzed 320 fungal extracts and their effect on the viability of a human neuronal cell line (SH-SY5Y), as well as their effects on the viability and functionality of the most effective compound on primary enteric neurons of murine origin. An extract from P. coprobium was identified to decrease viability with an EC50 of 0.23 ng/&micro

yearjournalcountryeditionlanguage
2019-07-30Molecules
10.3390/molecules24152776http://dx.doi.org/10.3390/molecules24152776