6533b7d7fe1ef96bd1268d91

RESEARCH PRODUCT

Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro

María Teresa DonatoJosé V. CastellMaría José Gómez-lechónAgustín Lahoz

subject

CYP2D6CYP2B6biologyBiochemistryCYP3A4ChemistryCYP1A2biology.proteinfood and beveragesCytochrome P450CYP2E1CYP2A6In vitro

description

Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy in combination with mass spectrometry is shown to be a robust, fast, and sensitive procedure for P450 activity assessment. This procedure allows a drastic reduction of the number of cells required in the assay and sample analysis time and increases throughput and reproducibility. Major applications of the probe cocktail strategy are P450 phenotyping of hepatocytes and induction studies.

yearjournalcountryeditionlanguage
2011-10-07
https://doi.org/10.1007/978-1-61779-367-7_7