6533b7d7fe1ef96bd1268e3d
RESEARCH PRODUCT
Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (T� 67)
James D. GriffinJames D. GriffinP WernetP WernetWolfgang OsterFriedhelm HerrmannR H MertelsmannK D SabbathK D Sabbathsubject
Rosette FormationErythroblastsmedicine.drug_classMonocyteAntibodies MonoclonalFluorescent Antibody TechniqueTransferrin receptorCell SeparationHematologyGeneral MedicineCell sortingBiologyFlow CytometryMonoclonal antibodyMolecular biologyHaematopoiesismedicine.anatomical_structurehemic and lymphatic diseasesReceptors TransferrinMonoclonalmedicinebiology.proteinAntibodyInterleukin 3description
Anti-TU 67 is a murine monoclonal antibody that recognizes the transferrin receptor. With respect to hematopoietic cells TU 67 is expressed by human multipotent colony-forming cells (CFU-Mix), erythroid progenitor cells (BFU-E and CFU-E) and a fraction of granulocyte/monocyte colony forming cells, but is not expressed by mature hematopoietic cells including erythrocytes, platelets, lymphocytes, and peripheral blood myeloid cells. The TU 67-positive fraction of normal bone marrow, separated by fluorescence-activated cell sorting (FACS) or immune rosettes, contained 87% of the erythroid progenitor cells. Erythroid progenitor cells were enriched up to 50-fold by using a combination of monoclonal antibodies to deplete mature hematopoietic cells, followed by positive selection of BFU-E and CFU-E by TU 67 antibody.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-04-01 | Blut |