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RESEARCH PRODUCT
Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
Manuel HidalgoEduardo FerreroMartin AldasoroMiriam GranadoNuria FernándezJorge Luis FerreroJosé María VilaAngel Luis García-villalónOscar Garcia-villarLuis MongeMaría Dolores Mauriciosubject
Vascular Endothelial Growth Factor Amedicine.medical_specialtyContraction (grammar)Article SubjectMedicinaBradykininlcsh:MedicineBiologyGeneral Biochemistry Genetics and Molecular BiologyTyrosine phosphorylationchemistry.chemical_compoundOrgan Culture TechniquesInternal medicinemedicineHumansPhosphorylationTyrosineSodium orthovanadateMesenteric arteriesVascular Endothelial Growth Factor Receptor-1Neovascularization PathologicGeneral Immunology and Microbiologylcsh:RTyrosine phosphorylationGeneral MedicineMesenteric ArteriesGene Expression Regulation NeoplasticVascular endothelial growth factor AEndocrinologymedicine.anatomical_structurechemistryTyrosinePhosphorylationVanadatesColorectal NeoplasmsResearch Articledescription
The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-01-01 | BioMed Research International |