6533b7d7fe1ef96bd1268ee2

RESEARCH PRODUCT

Determination and separation of diarylheptanoids from alder growing in Latvia

Galina TelyshevaOskars BikovensLiga Roze

subject

Internal standardbiologyChemistryalnus species; bark extracts; diarylheptanoids; oregonin; 1; 7-bis-(3; 4-dihydroxyphenyl)-3- hydroxyheptane-5-O-β-D-xylopbiology.organism_classificationHigh-performance liquid chromatographyAlderQuantitative determinationvisual_artvisual_art.visual_art_mediumOrganic chemistryExtraction methodsBarkDiarylheptanoids

description

The composition of diarylhepatnoids fraction isolated from bark of two alder species (grey alder and black alder) was studied. The efficiency of three extraction methods used for isolation of diarylhepatnoids from alder bark was compared. Two diarylhepatnoids: 1,7-bis-(3,4-dihydroxyphenyl)-heptan- 3-one-5-O-β-D-xylopyronoside (oregonin) and 1,7-bis-(3,4-dihydroxyphenyl)-3-hydroxyheptane-5-O-β-D- xylopyranoside were isolated from the bark of grey alder. The phenolic components of the extracts were analyzed by high-performance liquid chromatography (HPLC). Quantitative determination of oregonin was performed using an internal standard method. The results obtained show that alder barks are rich source of diarylhepatnoids.

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