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RESEARCH PRODUCT
Comparison of basal cytotoxicity of seven carbamates in CHO-K1 cells
Lina E FestilaM. FernándezMaría José Ruizsubject
CarbamateNeutral redAldicarbHealth Toxicology and Mutagenesismedicine.medical_treatmentChinese hamster ovary cellPropoxurBiologyPirimicarbPollutionchemistry.chemical_compoundchemistryBiochemistrymedicineEnvironmental ChemistryMTT assayCytotoxicitydescription
The cytotoxic effects of seven carbamate pesticides, aldicarb, aldicarb sulfone, aldicarb sulfoxide, benfuracarb, pirimicarb, propoxur and thiobencarb, were compared in Chinese hamster ovary (CHO-K1) cell line of Circetulus griseus. The endpoints evaluated were lysosomal function by neutral red assay and mitochondrial integrity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay). The carbamates tested were evaluated in both serum-free medium and in serum-containing medium. Results demonstrate that CHO-K1 lysosomes appeared more susceptible to propoxur, aldicarb and its metabolites than mitochondria. Aldicarb was the most toxic carbamate pesticide tested on CHO-K1 cells and aldicarb sulfoxide the least. The presence of foetal calf serum (FCS) diminished significantly the cytotoxicity of tested carbamates.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-04-01 | Toxicological & Environmental Chemistry |