6533b7d8fe1ef96bd1269903

RESEARCH PRODUCT

Conservation of amino-acid chromatograms.

E. KawerauT. Wieland

subject

chemistry.chemical_classificationchemistry.chemical_compoundPigmentChromatographyMultidisciplinaryChromatographychemistryNinhydrinQuantitative assayvisual_artvisual_art.visual_art_mediumAmino AcidsAmino acid

description

IN filter-paper partition chromatography, the ninhydrin reaction has been used almost exclusively for the identification and quantitative assay of amino-acids. One of the chief disadvantages of the blue pigment formed in this reaction is its rapid fading on filter-paper chromatograms. This makes it difficult to construct permanent or semi-permanent reference maps from which not only the type of amino-acid, but also the approximate quantity of the unknown mixture, can be deduced.

10.1038/168077b0https://pubmed.ncbi.nlm.nih.gov/14852959