6533b7d8fe1ef96bd12699d6
RESEARCH PRODUCT
Insulin and IGFs induce apoptosis in chick embryo retinas deprived of L-glutamine
Giovanni TesoriereMaria CarabillòMarianna LauricellaHelen MainE. GerbinoRenza VentoGiuseppe Calvarusosubject
Programmed cell deathChemistryInsulinmedicine.medical_treatmentApoptotic DNA fragmentationEmbryoRetinalCell BiologyAnatomyCycloheximideCell biologyGlutaminechemistry.chemical_compoundApoptosismedicineInsulin apoptosis chick embryo retina development growth factorsMolecular Biologydescription
In chick embryo retinas, cultured in serum-free medium lacking L-glutamine, IGF-I, IGF-II and insulin induced apoptotic DNA fragmentation and cell death, IGF-I being the most efficacious compound. The apoptotic effect, which was particularly evident in retinas removed from 7-day-old chick embryos, declined with the age of the embryos and disappeared after day 11. Apoptosis appeared after a time lag of 8 h and then increased with time up to 16 h. Cycloheximide, an inhibitor of protein synthesis, was capable of entirely abolishing apoptotic cell death. The effect induced by IGFs or insulin was suppressed by the addition of glutamine. Cytokine-mediated apoptosis was also observed after withdrawal of phosphate. We suggest that IGFs or insulin may produce, in retinas cultured in medium lacking L-glutamine or phosphate, a conflict of signals that could be lethal for retinal cells. Keywords:
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-07-29 |