Influence of photo-initiators in the preparation of methacrylate monoliths into poly(ethylene-co-tetrafluoroethylene) tubing for microbore HPLC.

6533b7d8fe1ef96bd1269a52

RESEARCH PRODUCT

Influence of photo-initiators in the preparation of methacrylate monoliths into poly(ethylene-co-tetrafluoroethylene) tubing for microbore HPLC.

Ernesto F. Simó-alfonso José Manuel Herrero-martínez Mónica Catalá-icardo S. Torres-cartas

subject

Ultraviolet Rays Morpholines 02 engineering and technology Poly(ethylene-co-tetrafluoroethylene)Methacrylate 01 natural sciences Biochemistry Analytical Chemistry Polymerization chemistry.chemical_compound ETFE Polymethacrylic Acids Photografting QUIMICA ANALITICA Nitriles Environmental Chemistry Reversed-phase liquid chromatography Polytetrafluoroethylene Spectroscopy Alkyl Chromatography High Pressure Liquid chemistry.chemical_classification Propiophenones Chemistry Herbicides Phenylurea Compounds 010401 analytical chemistry Acetophenones Caseins Polymer monolith Polymer Reversed-phase chromatography 021001 nanoscience & nanotechnology Photo-initiator Peptide Fragments 0104 chemical sciences Polymerization Photografting Methacrylates Tetrafluoroethylene 0210 nano-technology Nuclear chemistry

description

[EN] In this study, poly(butyl methacrylate-co-ethyleneglycol dimethacrylate) polymeric monoliths were in situ developed within 0.75 mm i.d. poly(ethylene-co-tetrafluoroethylene) (ETFE) tubing by UV polymerization via three different free-radical initiators fscce-azobisisobutyronitrile (AIBN), 2,2-dimethoxy-2-phenylacetophenone (DMPA) and 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMPP). The influence of the nature of each photo-initiator and irradiation time on the morphological features of the polymer was investigated by scanning electron microscopy, and the chromatographic properties of the resulting microbore columns were evaluated using alkyl benzenes as test substances. The beds photo-initiated with MTMPP gave the best performance (minimum plate heights of 38 mu m for alkyl benzenes) and exhibited a satisfactory reproducibility in the chromatographic parameters (RSD < 11%). These monolithic columns were also successfully applied to the separation of phenylurea herbicides, proteins and a tryptic digest of beta-casein. (C) 2019 Elsevier B.V. All rights reserved.

year	journal	country	edition	language
2020-01-01	Analytica chimica acta

10.1016/j.aca.2019.09.055 https://pubmed.ncbi.nlm.nih.gov/31735210