6533b7d8fe1ef96bd126a2a0
RESEARCH PRODUCT
Determination of busulfan in human plasma using high-performance liquid chromatography with pre-column derivatization and fluorescence detection.
Victoria CastelAmparo VerdeguerF Torres-molinaSara EsteveJosé Esteban PerisJosé-antonio Latorresubject
ChromatographyChemistryExtraction (chemistry)Reproducibility of ResultsGeneral ChemistryReference StandardsHigh-performance liquid chromatographyFluorescenceSensitivity and SpecificityFluorescence spectroscopyPentanechemistry.chemical_compoundSpectrometry FluorescenceCalibrationmedicineHumansDerivatizationQuantitative analysis (chemistry)Antineoplastic Agents AlkylatingBusulfanBusulfanChromatography High Pressure Liquidmedicine.drugdescription
A rapid, sensitive and reproducible high-performance liquid chromatographic assay for busulfan in human plasma was developed. After extraction of plasma samples with acetonitrile and methylene chloride, busulfan and the internal standard [1,5-bis(methanesulfonyloxy)pentane] were derivatized with 8-mercaptoquinoline to yield fluorescent compounds which were detected with a fluorescence detector equipped with filters of 360 nm (excitation) and 425 nm (emission). Calibration graphs showed a linear correlation (r>0.9990) over the concentration range of 20-2000 ng/ml. The recovery of busulfan from plasma standards was 70+/-5%. The detection and quantification limits for busulfan in plasma samples were established at 9 ng/ml and 20 ng/ml, respectively. The intra- and inter-assay variations were lower than 8% and 10%, respectively. The applicability of the method was verified by analyzing the plasma concentrations of busulfan in a patient to whom it was administered orally on two different days.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-06-01 | Journal of chromatography. B, Biomedical sciences and applications |