6533b7d8fe1ef96bd126a384
RESEARCH PRODUCT
Cloning, structure, cellular localization, and possible function of the tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila melanogaster.
Ansgar SchmidtStephan DreschersThomas KlöcknerGeorges Kaufer-stillgerJan-erik EdströmElisabeth GateffLutz KonradGerhard Beckersubject
DNA ComplementaryTumor suppressor geneMolecular Sequence DataMalignant transformationGene expressionAnimalsGenes Tumor SuppressorAmino Acid SequenceRNA MessengerCloning MolecularMolecular BiologyGeneCellular localizationAllelesCloningBlood CellsbiologyBase SequenceChromosome MappingCell Biologybiology.organism_classificationMolecular biologyCell Transformation NeoplasticDrosophila melanogasterLoricrinDrosophila melanogasterDevelopmental Biologydescription
The tumor suppressor gene, lethal(3)malignant blood neoplasm-1+, of Drosophila melanogaster is required for the differentiation of the phagocytic blood-cell type, the plasmatocyte. In the homozygously mutated state it causes the malignant transformation of these blood cells. We present here the cloning, sequencing, structure, and expression of the l(3)mbn-1+ gene during development. The cloned gene was identified by germ-line transformation, generation of revertants, and the detection of the corresponding mRNA in blood cells and other tissues. Homologies of the G-S-rich C-terminus of the putative MBN83 protein to human cytokeratins K1, K10, and mouse loricrin were found. The structure and possible function of the wild-type l(3)mbn-1+ gene are discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-05-01 | Developmental biology |