6533b7d8fe1ef96bd126a3c6
RESEARCH PRODUCT
ISOLATION OF RAT CDNA CLONES CODING FOR THE AUTOANTIGEN SS-B/LA - DETECTION OF SPECIES-SPECIFIC VARIATIONS
Holger BartschHelmut TrösterGabor L. IgloiMichael BachmannImre SemseiMartin Schwemmlesubject
PolyadenylationMolecular Sequence DataAutoantigensSingle-stranded binding proteinSpecies SpecificityComplementary DNASequence Homology Nucleic AcidGeneticsAnimalsHumansGenomic libraryAmino Acid SequenceCloning MolecularRibonucleoproteinGeneticsbiologyBase SequenceC-terminusNucleic acid sequenceGenetic VariationGeneral MedicineDNAMolecular biologyIntronsRatsRibonucleoproteinsGenBankbiology.proteindescription
Clones of cDNA coding for the autoantigen La (or SS-B) were isolated from a library made from rat liver. A comparison of the rat La cDNA (encoding from nt 38 to 1281 for rat La protein) with the sequences known for human and bovine La protein resulted in the identification of species-specific inserts. The inserts seem to be the result of multiplication of flanking sequences during evolution. In addition to these variations, we observed that rat La cDNAs exhibit non-canonical polyadenylation sites. Finally, a databank search resulted in the identification of a DNA sequence originally termed as TAG or TSG20X (GenBank accession No. X61893) which represents the C terminus of mouse La/SS-B protein.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-04-30 |