6533b7d8fe1ef96bd126ad7f
RESEARCH PRODUCT
Absence of binding of human salivary glycoprotein to human gingival fibroblast-like cells in vitro.
Andrej ZentnerT.g. Heaneysubject
chemistry.chemical_classificationChemistryGingivaMucinsPlasma protein bindingFibroblastsBlood Physiological PhenomenaGroup AMolecular biologyIn vitroCell Linemedicine.anatomical_structureAntigenCell culturemedicineCell AdhesionHumansGingival fibroblastSalivary Proteins and PeptidesGlycoproteinFibroblastSalivaGeneral DentistryProtein Bindingdescription
The aim of this study was to determine whether human high molecular weight salivary glycoprotein binds in vitro to human gingival fibroblast-like cells. Primary monolayer cultures of 2 human gingival fibroblast-like cell lines were incubated with a high molecular weight fraction of salivary glycoprotein which expressed blood group A activity and glycoprotein-cell binding probed using an FITC-conjugated mouse monoclonal antibody to human blood group A antigen. Surface fluorescence of protein-treated cells was found to be no greater than that of untreated or serum-treated control cultures. As significant binding of salivary glycoprotein to gingival fibroblast-like cells does not occur in vitro, saliva-mediated inhibition of fibroblast attachment to hydroxyapatite is not dependent on specific ligand-lectin interactions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-10-01 | European journal of oral sciences |