6533b7d8fe1ef96bd126b5c9
RESEARCH PRODUCT
Super-resolved linear fluorescence localization microscopy using photostable fluorophores: A virtual microscopy study
Udo BirkUdo BirkChristoph CremerAleksander Szczureksubject
0301 basic medicineMaterials sciencebusiness.industryMultispectral imageResolution (electron density)02 engineering and technology021001 nanoscience & nanotechnologyFluorescenceAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic Materials03 medical and health sciences030104 developmental biologyOpticsMicroscopyCalibrationPhotoactivated localization microscopyElectrical and Electronic EngineeringPhysical and Theoretical Chemistry0210 nano-technologybusinessVirtual microscopyVisible spectrumdescription
Abstract Current approaches to overcome the conventional limit of the resolution potential of light microscopy (of about 200 nm for visible light), often suffer from non-linear effects, which render the quantification of the image intensities in the reconstructions difficult, and also affect the quantification of the biological structure under investigation. As an attempt to face these difficulties, we discuss a particular method of localization microscopy which is based on photostable fluorescent dyes. The proposed method can potentially be implemented as a fast alternative for quantitative localization microscopy, circumventing the need for the acquisition of thousands of image frames and complex, highly dye-specific imaging buffers. Although the need for calibration remains in order to extract quantitative data (such as the number of emitters), multispectral approaches are largely facilitated due to the much less stringent requirements on imaging buffers. Furthermore, multispectral acquisitions can be readily obtained using commercial instrumentation such as e.g. the conventional confocal laser scanning microscope.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-12-01 | Optics Communications |