6533b7d8fe1ef96bd126b5eb
RESEARCH PRODUCT
Lignans from Torreya jackii identified by stopped-flow high-performance liquid chromatography–nuclear magnetic resonance spectroscopy
Yu ZhaoXianfeng SunJoachim StöckigtBettina SchmittAslieh NookandehBernd Schneidersubject
ChromatographybiologyElutionChemistryOrganic ChemistryAnalytical chemistryGeneral MedicineNuclear magnetic resonance spectroscopybiology.organism_classificationMass spectrometryBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryTorreya jackiiProton NMRTaxaceaeSpectroscopydescription
Abstract Coupled reversed-phase HPLC–NMR spectroscopy has been applied to the rapid detection and identification of plant metabolites of Torreya jackii , a species of Taxaceae. Analysis consisted of gradient HPLC elution and directly coupled 1 H NMR (500 MHz) spectroscopic detection in a stopped-flow mode. Seven lignans were detected and their structures were elucidated, based on their HPLC– 1 H NMR spectra and MS data. The structures were confirmed by isolation of the single components followed by conventional NMR measurements.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-04-01 | Journal of Chromatography A |