6533b7d8fe1ef96bd126b6e2
RESEARCH PRODUCT
Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum
Maria Rita SchiavoGiuseppe CalvarusoRenza VentoGennaro TaibiMichela GiulianoGiovanni Tesorieresubject
DNA ReplicationThymidine kinase activityDNA synthesisEmbryoBlood ProteinsBiologyMolecular biologyGrowth InhibitorsRetinaIn vitroDithiothreitolMolecular Weightchemistry.chemical_compoundOrgan Culture TechniquesDevelopmental NeurosciencechemistryBiochemistrySephadexAnimalsCattlechick embryoFetal bovine serumDNADevelopmental Biologydescription
Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-05-01 | Developmental Brain Research |