6533b7d9fe1ef96bd126c134

RESEARCH PRODUCT

Determination of organic and inorganic anions in insect haemolymph and midgut contents by ion chromatography

R. MartínezE. AlcácerAgustín PastorCarmen ForcadaMaría Dolores Garcerá

subject

ChromatographyConductometrybiologyfungiOrganic ChemistryIon chromatographyGeneral MedicinePhosphateBiochemistryChlorideAnalytical ChemistryPhthalic acidchemistry.chemical_compoundchemistrymedicinebiology.proteinCarboxylateQuantitative analysis (chemistry)medicine.drugOrganic anion

description

A simple and rapid ion-exchange chromatographic method with conductimetric detection for the determination of carboxylic acids (succinic, malic and citric) and inorganic anions (chloride, phosphate and sulphate) in haemolymph and midgut content of Lepidoptera larvae is provided. The mobile phase is 0.975 mM phthalic acid at pH 4.1. The procedure of sample extractions was simple. Comparison of the results with whose reported in the bibliography showed that the values were similar. The ion-exchange chromatographic–conductimetric detection method permits the analysis of various organic and inorganic anions in small biological samples.

yearjournalcountryeditionlanguage
1997-11-01Journal of Chromatography A
https://doi.org/10.1016/s0021-9673(97)00702-4