6533b7d9fe1ef96bd126c279

RESEARCH PRODUCT

Separation of deoxyribonucleases (DNases) of normal human stratum corneum and psoriatic scales by micro-disc-electrophoresis.

E. J. ZöllnerF J FörsterRudolf K. ZahnG. Leonhardi

subject

DermatologyKeratinStratum corneummedicineHumansPsoriasisCentrifugationPolyacrylamide gel electrophoresisSkinDifferential centrifugationchemistry.chemical_classificationChromatographyDeoxyribonucleasesintegumentary systembiologyChemistryGeneral MedicineHydrogen-Ion ConcentrationEnzyme assayIsoenzymesMolecular WeightElectrophoresismedicine.anatomical_structurebiology.proteinElectrophoresis Polyacrylamide GelDeoxyribonucleasesSubcellular Fractions

description

Normal stratum corneum and psoriatic scales were homogenized and a differential centrifugation was performed. The DNase activity of the individual fractions was investigated by micro-disc-electrophoresis. At pH 5 only in the 600 × g pellet and 105.000 × g supernatant of normal keratin DNase activity could be observed. However, all psoriatic fractions showed distinct enzyme activities. At pH 7.4 little psoriatic DNase activity could only be demonstrated in the 105.000 × g supernatant. Except from the 15.000 × g pellet all fractions of normal stratum corneum displayed marked activities. In addition the 105.000 × g supernatant showed two different DNase bands.

yearjournalcountryeditionlanguage
1975-10-29Archives for dermatological research = Archiv fur dermatologische Forschung
10.1007/bf00561146https://pubmed.ncbi.nlm.nih.gov/974