6533b7d9fe1ef96bd126cbe6

RESEARCH PRODUCT

PCR ITS-RFLP for Penicillium Species and Other Genera

Sandrine RousseauxMichèle Guilloux-benatier

subject

0301 basic medicineGenetics03 medical and health sciencesfungi030106 microbiologyRestriction digestIdentification (biology)Restriction fragment length polymorphismBiologyPenicillium speciesSpecific identification

description

Among numerous molecular methodologies developed for highly specific identification of filamentous fungi isolates, here we describe restriction digestion analysis of the ITS products as an easy method to identify isolates of filamentous fungi. This technique is a rapid and reliable method appropriate for routine identification of filamentous fungi. This can be used to screen large numbers of isolates from various environments in a short time. The use of different endonucleases allowed generating individual restriction profiles. The individual profiles obtained were combined into composite restriction patterns characteristic of a species. Eleven different genera can be differentiated and among them 41 different species.

yearjournalcountryeditionlanguage
2016-12-07
https://doi.org/10.1007/978-1-4939-6707-0_21