6533b7d9fe1ef96bd126cd97
RESEARCH PRODUCT
Metabolic flux understanding of Pichia pastoris grown on heterogenous culture media
Jesús PicóJ.m. González-martínezJ.m. González-martínezMarta TortajadaAlberto FerrerAbel Folch-fortunyFrancisco Llanerassubject
Principal Component AnalysisbiologyMathematical modelManufacturing processComputer scienceProcess Chemistry and TechnologySystems biologyMonte Carlo samplingESTADISTICA E INVESTIGACION OPERATIVACellular functionsMetabolic networkMetabolic networkMissing-data methods for Exploratory Data AnalysisContext (language use)biology.organism_classificationINGENIERIA DE SISTEMAS Y AUTOMATICAComputer Science ApplicationsAnalytical ChemistryPichia pastorisEconometricsBiochemical engineeringPossibilistic consistency analysisFlux (metabolism)SpectroscopySoftwaredescription
[EN] Within the emergent field of Systems Biology, mathematical models obtained from physical chemical laws (the so-called first principles-based models) of microbial systems are employed to discern the principles that govern cellular behaviour and achieve a predictive understanding of cellular functions. The reliance on this biochemical knowledge has the drawback that some of the assumptions (specific kinetics of the reaction system, unknown dynamics and values of the model parameters) may not be valid for all the metabolic possible states of the network. In this uncertainty context, the combined use of fundamental knowledge and data measured in the fermentation that describe the behaviour of the microorganism in the manufacturing process is paramount to overcome this problem. In this paper, a grey modelling approach is presented combining data-driven and first principles information at different scales, developed for Pichia pastoris cultures grown on different carbon sources. This approach will allow us to relate patterns of recombinant protein production to intracellular metabolic states and correlate intra and extracellular reactions in order to understand how the internal state of the cells determines the observed behaviour in P. pastoris cultivations.
year | journal | country | edition | language |
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2014-05-15 |