6533b7d9fe1ef96bd126ce35

RESEARCH PRODUCT

Expression of the succinate dehydrogenase genes (sdhCAB) from the facultatively anaerobic paenibacillus macerans during aerobic growth

Jan SchirawskiGottfried UndenThomas Hankeln

subject

Paenibacillus maceranschemistry.chemical_classificationbiologySDHBSuccinate dehydrogenaseSDHAmacromolecular substancesGeneral MedicineFumarate reductasebiology.organism_classificationBiochemistryMicrobiologyPaenibacillusEnzymeBiochemistrychemistryGene clusterGeneticsbiology.proteinMolecular Biology

description

Paenibacillus (formerly Bacillus) macerans is capable of succinate oxidation under oxic conditions and fumarate reduction under anoxic conditions. The reactions are catalyzed by different enzymes, succinate dehydrogenase (Sdh) and fumarate reductase (Frd). The genes encoding Sdh (sdhCAB) were analyzed. The gene products of sdhA and sdhB were similar to the subunits of known Sdh and Frd enzymes. The hydrophobic subunit SdhC showed close sequence similarity to the class of Sdh/Frd enzymes containing diheme cytochrome b. From the sdhCAB gene cluster two transcripts were produced, one comprising sdhCAB, the other sdhAB. The transcripts were found only during aerobic growth, and the amount was directly proportional to Sdh activity, but inversely proportional to Frd activity.

yearjournalcountryeditionlanguage
1998-09-09Archives of microbiology
10.1007/s002030050646https://pubmed.ncbi.nlm.nih.gov/9732445