6533b7d9fe1ef96bd126ce59
RESEARCH PRODUCT
Rapid characterization of wild and collection strains of the genus Zygosaccharomyces according to mitochondrial DNA patterns
T. HuertaJosé Manuel GuillamónIgnacio Sánchezsubject
GeneticsMitochondrial DNAGenus ZygosaccharomycesZygosaccharomycesBiologybiology.organism_classificationDNA MitochondrialMicrobiologyHaeIIIRestriction enzymeEndonucleasechemistry.chemical_compoundSpecies SpecificitychemistrySaccharomycetalesFood MicrobiologyGeneticsmedicinebiology.proteinMolecular BiologyPolymorphism Restriction Fragment LengthDNAmedicine.drugdescription
Several wild and collection strains of the genus Zygosaccharomyces were characterized using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with three endonucleases (HaeIII, HinfI and RsaI) made it possible to differentiate each species and to identify the wild strains, isolated from the same spoiled concentrated must, as belonging to the species Z. rouxii. The HinfI restriction enzyme produced a strain-specific pattern which allowed us to recognize that the seven wild isolates belonged to only three strains.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-02-15 | FEMS Microbiology Letters |