6533b7dafe1ef96bd126d81f

RESEARCH PRODUCT

Mutation rate of bacteriophage ΦX174 modified through changes in GATC sequence context

Marianoel Pereira-gómezRafael SanjuánJosé M. Cuevas

subject

Microbiology (medical)Mutation rateGenome ViralDNA Mismatch RepairMicrobiologyGenomeEvolution MolecularBacteriophageGeneticsNucleotideMolecular BiologyEcology Evolution Behavior and SystematicsGeneticschemistry.chemical_classificationBase SequencebiologyDNA virusDNA Methylationbiology.organism_classificationInfectious DiseaseschemistrySingle Stranded DNA VirusDNA ViralMutationDNA mismatch repairBacteriophage phi X 174

description

Bacteriophage ΦX174 has a relatively high mutation rate of 10⁻⁶ substitutions per nucleotide per strand copying. A thirty-fold reduction in the mutation rate was achieved by introducing seven GATC sequences in its genome. This motif allows for methyl-directed mismatch repair and is strongly avoided in nature by ΦX174 and other phages.

yearjournalcountryeditionlanguage
2011-05-05Infection, Genetics and Evolution
https://doi.org/10.1016/j.meegid.2011.08.024